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mouse osteoblast-like cells 7f2  (BioResource International Inc)

 
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    BioResource International Inc mouse osteoblast-like cells 7f2
    In vitro uptake of DiI-labeled liposomes in <t>7F2</t> osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).
    Mouse Osteoblast Like Cells 7f2, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse osteoblast-like cells 7f2/product/BioResource International Inc
    Average 90 stars, based on 1 article reviews
    mouse osteoblast-like cells 7f2 - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health"

    Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

    Journal: Pharmaceuticals

    doi: 10.3390/ph15040490

    In vitro uptake of DiI-labeled liposomes in 7F2 osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).
    Figure Legend Snippet: In vitro uptake of DiI-labeled liposomes in 7F2 osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).

    Techniques Used: In Vitro, Labeling, Liposomes, Microscopy, Cell Culture, Fluorescence, Staining

    The effect of astaxanthin extract and asta-loaded liposomes on cell viability of 7F2 osteoblasts. 7F2 osteoblasts were treated with ( A ) astaxanthin extract and ( B ) empty and asta-loaded liposomes for 24 h. Cell viabilities of 7F2 osteoblasts were measured by MTT assay. The data are shown as the means ± standard deviation. (* p < 0.05 related to control; + p < 0.05 related to 2% ETOH; # p < 0.05 related to liposomes).
    Figure Legend Snippet: The effect of astaxanthin extract and asta-loaded liposomes on cell viability of 7F2 osteoblasts. 7F2 osteoblasts were treated with ( A ) astaxanthin extract and ( B ) empty and asta-loaded liposomes for 24 h. Cell viabilities of 7F2 osteoblasts were measured by MTT assay. The data are shown as the means ± standard deviation. (* p < 0.05 related to control; + p < 0.05 related to 2% ETOH; # p < 0.05 related to liposomes).

    Techniques Used: Liposomes, MTT Assay, Standard Deviation, Control

    The effect of asta-loaded liposomes on 7F2 osteoblast differentiation and mineralization. 7F2 osteoblasts were treated with 50 μg/mL of ascorbic and 10 mM β-glycerophosphate to induce osteoblast differentiation and mineralization. ( A ) Cells were then incubated in the presence or absence of asta-loaded liposomes for 1, 4 and 7 days. Results are revealed as a ratio with mean ± standard deviation ( n = 3). ( B ) ARS staining of calcium deposits on days 1, 7 and 14 (×100 magnification, scale bar = 200 nm). ( C ) Quantification of calcium deposits on days 1, 7 and 14. Calcium deposition was quantified by dissolving ARS aggregates into 10% cetylpyridinium chloride and evaluating the absorbance at 560 nm. Results are revealed as a ratio with mean ± standard deviation ( n = 3) (* p < 0.05 related to control group of the same day, # p < 0.05 related to MM of the same day, + p < 0.05 related to empty liposome treatment of the same day).
    Figure Legend Snippet: The effect of asta-loaded liposomes on 7F2 osteoblast differentiation and mineralization. 7F2 osteoblasts were treated with 50 μg/mL of ascorbic and 10 mM β-glycerophosphate to induce osteoblast differentiation and mineralization. ( A ) Cells were then incubated in the presence or absence of asta-loaded liposomes for 1, 4 and 7 days. Results are revealed as a ratio with mean ± standard deviation ( n = 3). ( B ) ARS staining of calcium deposits on days 1, 7 and 14 (×100 magnification, scale bar = 200 nm). ( C ) Quantification of calcium deposits on days 1, 7 and 14. Calcium deposition was quantified by dissolving ARS aggregates into 10% cetylpyridinium chloride and evaluating the absorbance at 560 nm. Results are revealed as a ratio with mean ± standard deviation ( n = 3) (* p < 0.05 related to control group of the same day, # p < 0.05 related to MM of the same day, + p < 0.05 related to empty liposome treatment of the same day).

    Techniques Used: Liposomes, Incubation, Standard Deviation, Staining, Control



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    BioResource International Inc mouse osteoblast-like cells 7f2
    In vitro uptake of DiI-labeled liposomes in <t>7F2</t> osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).
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    mouse osteoblast-like cells (7f2)  (BioResource International Inc)
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    BioResource International Inc mouse osteoblast-like cells (7f2)
    In vitro uptake of DiI-labeled liposomes in <t>7F2</t> osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).
    Mouse Osteoblast Like Cells (7f2), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse osteoblast-like cells (7f2)/product/BioResource International Inc
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    In vitro uptake of DiI-labeled liposomes in 7F2 osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).

    Journal: Pharmaceuticals

    Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

    doi: 10.3390/ph15040490

    Figure Lengend Snippet: In vitro uptake of DiI-labeled liposomes in 7F2 osteoblasts measured by fluorescent microscopy. The cells were cultured with DiI-labeled liposomes for 4 h. Thereafter, red fluorescence (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent microscopy equipped with a CCD system (×100 magnification, scale bar = 200 nm).

    Article Snippet: Mouse osteoblast-like cells (7F2) and mouse macrophage cells (Raw264.7) were obtained from Bioresource Collection and Research Center (BCRC), Taiwan.

    Techniques: In Vitro, Labeling, Liposomes, Microscopy, Cell Culture, Fluorescence, Staining

    The effect of astaxanthin extract and asta-loaded liposomes on cell viability of 7F2 osteoblasts. 7F2 osteoblasts were treated with ( A ) astaxanthin extract and ( B ) empty and asta-loaded liposomes for 24 h. Cell viabilities of 7F2 osteoblasts were measured by MTT assay. The data are shown as the means ± standard deviation. (* p < 0.05 related to control; + p < 0.05 related to 2% ETOH; # p < 0.05 related to liposomes).

    Journal: Pharmaceuticals

    Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

    doi: 10.3390/ph15040490

    Figure Lengend Snippet: The effect of astaxanthin extract and asta-loaded liposomes on cell viability of 7F2 osteoblasts. 7F2 osteoblasts were treated with ( A ) astaxanthin extract and ( B ) empty and asta-loaded liposomes for 24 h. Cell viabilities of 7F2 osteoblasts were measured by MTT assay. The data are shown as the means ± standard deviation. (* p < 0.05 related to control; + p < 0.05 related to 2% ETOH; # p < 0.05 related to liposomes).

    Article Snippet: Mouse osteoblast-like cells (7F2) and mouse macrophage cells (Raw264.7) were obtained from Bioresource Collection and Research Center (BCRC), Taiwan.

    Techniques: Liposomes, MTT Assay, Standard Deviation, Control

    The effect of asta-loaded liposomes on 7F2 osteoblast differentiation and mineralization. 7F2 osteoblasts were treated with 50 μg/mL of ascorbic and 10 mM β-glycerophosphate to induce osteoblast differentiation and mineralization. ( A ) Cells were then incubated in the presence or absence of asta-loaded liposomes for 1, 4 and 7 days. Results are revealed as a ratio with mean ± standard deviation ( n = 3). ( B ) ARS staining of calcium deposits on days 1, 7 and 14 (×100 magnification, scale bar = 200 nm). ( C ) Quantification of calcium deposits on days 1, 7 and 14. Calcium deposition was quantified by dissolving ARS aggregates into 10% cetylpyridinium chloride and evaluating the absorbance at 560 nm. Results are revealed as a ratio with mean ± standard deviation ( n = 3) (* p < 0.05 related to control group of the same day, # p < 0.05 related to MM of the same day, + p < 0.05 related to empty liposome treatment of the same day).

    Journal: Pharmaceuticals

    Article Title: Development of Astaxanthin-Loaded Nanosized Liposomal Formulation to Improve Bone Health

    doi: 10.3390/ph15040490

    Figure Lengend Snippet: The effect of asta-loaded liposomes on 7F2 osteoblast differentiation and mineralization. 7F2 osteoblasts were treated with 50 μg/mL of ascorbic and 10 mM β-glycerophosphate to induce osteoblast differentiation and mineralization. ( A ) Cells were then incubated in the presence or absence of asta-loaded liposomes for 1, 4 and 7 days. Results are revealed as a ratio with mean ± standard deviation ( n = 3). ( B ) ARS staining of calcium deposits on days 1, 7 and 14 (×100 magnification, scale bar = 200 nm). ( C ) Quantification of calcium deposits on days 1, 7 and 14. Calcium deposition was quantified by dissolving ARS aggregates into 10% cetylpyridinium chloride and evaluating the absorbance at 560 nm. Results are revealed as a ratio with mean ± standard deviation ( n = 3) (* p < 0.05 related to control group of the same day, # p < 0.05 related to MM of the same day, + p < 0.05 related to empty liposome treatment of the same day).

    Article Snippet: Mouse osteoblast-like cells (7F2) and mouse macrophage cells (Raw264.7) were obtained from Bioresource Collection and Research Center (BCRC), Taiwan.

    Techniques: Liposomes, Incubation, Standard Deviation, Staining, Control